Journal: Journal of Nanobiotechnology

Article Title: T7 peptide-engineered liposomal Irisin mitigates PND progression through AMPK/PGC-1α signaling: multi-omic evidence of metabolic and epigenetic modulation

doi: 10.1186/s12951-026-04109-7

Figure Lengend Snippet: Neuron Membrane Fusion Enhances Liposomal Order and Surface Exposure of T7 Peptides. Note: ( A ) Raman spectroscopy showing enhanced symmetric stretching peaks of lipid chains in neuron-mimetic liposomes, indicating improved membrane order; ( B ) FITC-T7 peptide quenching assay demonstrating that, after membrane fusion, T7 peptides remain exposed on the particle surface with high accessibility; ( C ) Western blot analysis confirming the presence of neuronal membrane markers Na + /K + -ATPase and NCAM in fused liposomes; ( D ) TEM image showing that neuron membrane-fused liposomes exhibit a well-defined outer membrane and regular morphology. ** p < 0.01

Article Snippet: Liposome samples were dropped onto CaF 2 slides and air-dried before analysis with a confocal Raman spectrometer (Renishaw inVia Reflex, UK) using a 785 nm excitation wavelength.

Techniques: Membrane, Raman Spectroscopy, Liposomes, Western Blot

Journal: Pharmaceutical Research

Article Title: Impact of Different Packaging Configurations on A Topical Cream Product

doi: 10.1007/s11095-024-03772-5

Figure Lengend Snippet: Confocal Raman microscopy analysis of acyclovir cream formulations from different packaging configurations and dispensing methods. Light microscopic images and corresponding Raman false color images of the areas indicated by the red square of samples dispensed from the tube ( A ), dispensed from the pump through the nozzle ( B ) and a sample removed from the inside of the pump container closure system without dispensing through the nozzle ( C ). The false color images show the cream base (black), acyclovir crystals (yellow) and dimethicone globules (blue): and the corresponding single spectra of these components (D).

Article Snippet: For Raman analysis, samples of the acyclovir formulations from the tube and pump before and after dispensing were evenly spread on a CaF 2 glass slide and examined using a confocal Raman microscope (alpha300 R + , WITec GmbH, Ulm, Germany).

Techniques: Microscopy, Cream

Journal: Cellular and Molecular Life Sciences

Article Title: The distinct phenotype of primary adipocytes and adipocytes derived from stem cells of white adipose tissue as assessed by Raman and fluorescence imaging

doi: 10.1007/s00018-022-04391-2

Figure Lengend Snippet: The flow scheme showing the isolation of primary adipocytes and procedures necessary to obtain culture of SVF-derived adipocytes. Samples of the interscapular (iBAT) and epididymal white adipose tissue (eWAT) were isolated from C57Bl/6J mice at the age of 10 weeks, then subjected to the procedures outlined above. Cell culture procedure was performed according to the literature

Article Snippet: Raman spectra of cultured cells were measured on CaF 2 slides with the application of the 60 × water immersion objective (NA = 1.0, Zeiss Fluor, Germany) and for the primary adipocytes using a 20 × air objective (NA = 0.45, Nikon CFI S Plan Fluor ELWD, Japan) due to the different sample preparation technique.

Techniques: Isolation, Derivative Assay, Cell Culture

Journal: Cellular and Molecular Life Sciences

Article Title: The distinct phenotype of primary adipocytes and adipocytes derived from stem cells of white adipose tissue as assessed by Raman and fluorescence imaging

doi: 10.1007/s00018-022-04391-2

Figure Lengend Snippet: Distinct changes of UCP-1 and perilipin-1 expression in preadipocytes and SVF-derived adipocytes. Representative images ( A ) of cells from simultaneously stained for UCP-1 (red fluorescence) and perilipin-1 (green fluorescence). The quantitative analysis of ratios of positively stained UCP-1 or perilipin-1 areas to the total area of the cell ( B ). Values shown in box plots: mean (horizontal line), SD (box), minimal and maximal values (whiskers). Statistical significance * p < 0.05

Article Snippet: Raman spectra of cultured cells were measured on CaF 2 slides with the application of the 60 × water immersion objective (NA = 1.0, Zeiss Fluor, Germany) and for the primary adipocytes using a 20 × air objective (NA = 0.45, Nikon CFI S Plan Fluor ELWD, Japan) due to the different sample preparation technique.

Techniques: Expressing, Derivative Assay, Staining, Fluorescence

Journal: Cellular and Molecular Life Sciences

Article Title: The distinct phenotype of primary adipocytes and adipocytes derived from stem cells of white adipose tissue as assessed by Raman and fluorescence imaging

doi: 10.1007/s00018-022-04391-2

Figure Lengend Snippet: Differences in the chemical composition of primary and SVF-derived mature adipocytes. Microscopic and Raman images of primary and SVF-derived mature adipocytes (day 7 after MIII) for eWAT and iBAT with averaged Raman spectra ( A , B ). Degree of lipid unsaturation (based on I 1656 /I 1442 ) determined for each AT depot and presented in box plots (the number of C=C double bonds was based on the calibration curve, C ). Values shown in box plots: mean (horizontal line), SD (box), minimal and maximal values (whiskers). Statistical significance * p < 0.05

Article Snippet: Raman spectra of cultured cells were measured on CaF 2 slides with the application of the 60 × water immersion objective (NA = 1.0, Zeiss Fluor, Germany) and for the primary adipocytes using a 20 × air objective (NA = 0.45, Nikon CFI S Plan Fluor ELWD, Japan) due to the different sample preparation technique.

Techniques: Derivative Assay